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請輸入基因編號或名稱:
產品分類:

 

 

載體構建服務

 

權陽生物開發了無縫隙連接(Seamlesscloning)技術,不需要酶切位點,可以把任意酶切位點連接在一起,在連接處不留下多余的序列,我們擁有300多種商品化載體,包括原核表達載體,哺乳動物表達載體,線蟲表達載體,病毒載體,報告基因檢測載體,大多數載體可免費提供給客戶使用!

 

服務范圍:

 

  • 1)哺乳動物ORF表達克隆構建;
  •  
  • 2)慢病毒、腺病毒載體構建;
  •  
  • 3)構建shRNA, RNAi 或 miRNA 表達載體構建;
  •  
  • 4)構建knock out 或knock in 質粒;
  •  
  • 5)gRNA載體構建;
  •  
  • 6)原核表達載體構建;
  •  
  • 7)酵母表達載體構建;
  •  
  • 8)突變質粒構建。
  •  

服務流程:

所以載體都經過測序驗證,保證100% 正確。給客戶提供原始測序結果和質粒圖譜等COA文件。 

本公司免費提供各種常用的載體,包括通用克隆載體,表達載體等,具體有需要請咨詢,我們沒有的載體需客戶提供,同時需要提供載體信息

 

提供產品


  •   》 重組質粒(3-5ug)

  •   》甘油菌(500ul)

  •   》COA文件


COA文件樣式(例):



Construct Information
Gene Name RBMS3(NM_001003793) Gene Length 1323bp
Cloning Vector pUC57-Amp Cloning Strategy HindIII and XhoI
Construct Resistant Ampicillin in E.coli Construct ID CN363-B2958
QC Items
QC Items Method Specifications Results
Target Sequence Sequence alignment Sequencing results are consistent to the confirmed sequence. Pass
Vector Sequence Sequence alignment 20bp flanking sequences of the vector are correct. NA
Reading Frame Sequence alignment Frame is correct and consistent to the client's requirement. Pass
Fragment Size Restriction Digests The size of inserted fragment is right and free of any contaminated bands. Pass
Quality/Quantity UV spectrophotometry Miniprep: OD260/280=1.7~2.0
5µg/tube, 1 tube,  Lyophilized
Pass
Appearance Visual inspection Clear and free of foreign particles. Pass
Customized Test NA NA NA
Restriction Digestion Map
  M 1 2  
 
Lane M: DNA Marker
 
Lane 1: Plasmid digested by EcoRI
 
Lane 2: Plasmid DNA 

 
Certified by:    Huanhuan Han      Date:   02/11/2015

 
測序結果(例)




 
1.質量保證 2.發貨時間 3.關于售后
Transheep 有一套成熟的質檢程序,產品發貨前均經過質量檢測,確保您收到的產品質量無誤! Transheep擁有大量的基因現貨產品,本公司的現貨產品從訂貨日期起一周以內即可到貨,大大縮短了您的等待時間!另外Transheep的一站式克隆平臺也能讓您體驗到前所未有的快速定制服務! Transheep所售出的產品全部登記在冊,如果您拿到我們的產品覺得和實質不相符或存在問題,您只需要告訴我們相關產品信息我們會迅速做出反應,為您提供解決方案或退款!
4.訂購流程 5.聯系我們 6.相關服務:
聯系我們→客戶或銷售人員與您溝通→簽訂合同→支付預付款→執行項目→確認收貨→支付尾款→售后服務 Transheep提供免費的技術咨詢,任何關于產品或者技術問題,都可以聯系我們們。 免費熱線:400-0179890 服務郵箱:[email protected] 基因合成
亞克隆構建
3,UTR驗證實驗
啟動子合成及驗證
慢病毒包裝
腺病毒包裝
穩轉株構建



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